Download PDF by Xiaoshi Wang: A Novel Heme-Thiolate Peroxygenase AaeAPO and Its

By Xiaoshi Wang

ISBN-10: 3319032356

ISBN-13: 9783319032351

during this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly came across heme-thiolate peroxygenase, AaeAPO. This enzyme category comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings makes a speciality of an extracellular P450 enzyme which isn't restricted in its balance and shortage of solubility and consequently is correct for common commercial use. the writer demonstrates that the peroxygenase catalyzes quite a lot of reactions. from time to time the writer even describes very tough alterations in molecules which are hugely inert. Her particular investigations offer a mechanistic framework for a way the peroxygenase catalyzes this kind of huge variety of reactions. a massive spotlight of this thesis is the id of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing swift kinetic and spectroscopic equipment, in addition to the elucidation of the thermodynamic homes of those high-energy intermediates. This paintings provides new perception into an immense type of enzymes.

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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry (Springer Theses) by Xiaoshi Wang


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